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Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and
A Revoir1, D J Ballard1, D Syndercombe Court1
1King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
Abstract:
Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.
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