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Related Experiment Video

Updated: May 3, 2026

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Print-to-print: printer-enabled out-of-cleanroom multiobject microprinting method.

Siyuan Xing1, Siwei Zhao1, Tingrui Pan1

  • 1Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, California, USA.

Methods in Cell Biology
|January 21, 2014
PubMed
Summary

A new Print-to-Print (P2P) method enables easy micropatterning for biological research using a standard printer and superhydrophobic films. This accessible technique supports high-throughput, quantitative studies on miniature biological objects without expensive equipment.

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Area of Science:

  • * Engineering
  • * Biology
  • * Materials Science

Background:

  • * Micropatterning techniques are crucial for high-throughput, quantitative biological research.
  • * Existing methods often require expensive equipment and cleanroom access, limiting their use in standard labs.
  • * There is a need for accessible, versatile micropatterning solutions for biological applications.

Purpose of the Study:

  • * To present a simple, versatile microprinting process called Print-to-Print (P2P).
  • * To enable the formation of multiobject micropatterns for biological applications.
  • * To demonstrate an accessible alternative to existing micropatterning technologies.

Main Methods:

  • * Utilized a standard solid-phase printer and custom-made superhydrophobic (SH) films.
Keywords:
Bio-fabricationBio-patterningBio-printingMicrofabricationMicropatterningRapid-prototyping

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  • * Employed a noncontact droplet transferring and printing process.
  • * No thermal or chemical treatments were involved in the printing process.

    • Main Results:

    • * Achieved a minimal feature resolution of 229 ± 17 μm.
    • * Successfully applied the P2P process to various common biological substrates.
    • * Demonstrated the ability to create multiobject co-patterns.
    • * Showcased the reusability of the superhydrophobic substrates.

    Conclusions:

    • * The Print-to-Print (P2P) process offers a simple, versatile, and accessible method for micropatterning.
    • * This technique is suitable for sensitive biological applications due to its noncontact nature.
    • * P2P technology can be applied to diverse substrates and for creating complex co-patterns, with reusable substrates.
    • * P2P provides a cost-effective solution for high-throughput biological investigations.