Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metagenome-assembled genomes from biological soil crusts in sandy sediments of Kitty Todd Nature Preserve, OH, USA.

Microbiology resource announcements·2026
Same author

Whole genome sequencing of three cloacal bacterial isolates from migratory birds.

Microbiology resource announcements·2026
Same author

Whole-genome sequencing and annotation of six metallotolerant bacterial isolates recovered from a retention pond at the Rochester Institute of Technology, New York.

Microbiology resource announcements·2026
Same author

Cerium as corrosion inhibitor for Sn-3Ag-0.5Cu solder alloy in 3.5% NaCl solution.

Scientific reports·2026
Same author

Inhibition of endocytosis by glycans arises from steric rather than electrostatic repulsion.

Biophysical journal·2026
Same author

Whole-genome sequencing of two endophytic bacteria from immature cones of Northern white cedar (<i>Thuja occidentalis</i>).

Microbiology resource announcements·2026
Same journal

Oral colon-targeted micro-nano formulation engineered in microfluid for synergistic therapy of inflammatory bowel disease.

Biomaterials·2026
Same journal

Manganese@Gold cluster-coordinated covalent organic frameworks-based artificial metalloenzymes with cascade biocatalysis and amplified systemic stimulation to combat malignant tumor metastasis.

Biomaterials·2026
Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

12.8K

Measuring stem cell dimensionality in tissue scaffolds.

Tanya M Farooque1, Charles H Camp1, Christopher K Tison1

  • 1Biosystems & Biomaterials Division, National Institute of Standards & Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.

Biomaterials
|January 21, 2014
PubMed
Summary
This summary is machine-generated.

Scaffolds promote more three-dimensional (3D) cell shapes compared to flat surfaces. Scaffold properties can be tailored to control cell dimensionality for better tissue engineering and in vitro models.

Keywords:
3D cell cultureBone marrow stromal cellCell shapeCell-material interactionsNanofiberTissue engineering

More Related Videos

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

9.8K
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.8K

Related Experiment Videos

Last Updated: May 3, 2026

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

12.8K
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

9.8K
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.8K

Area of Science:

  • Tissue Engineering
  • Biomaterials Science
  • Cell Biology

Background:

  • Scaffold systems are crucial for creating 3D microenvironments in tissue engineering and in vitro models.
  • Cell morphology is intrinsically linked to cell function, making control over cell shape vital.
  • Understanding how scaffold properties influence cell dimensionality is key for directing cellular behavior.

Purpose of the Study:

  • To test the hypothesis that cells adopt more 3D morphologies in scaffolds versus planar substrates.
  • To quantify the influence of different scaffold properties on cell shape and dimensionality.
  • To develop a method for assessing scaffold-induced changes in cell morphology.

Main Methods:

  • Primary human bone marrow stromal cells (hBMSCs) were cultured on six distinct scaffolds and a 2D planar control.
  • 3D confocal microscopy and 3D image analysis were employed to assess hBMSC shape and dimensionality.
  • A 'Dimensionality Matrix' and Z-Depth measurements were used to classify and quantify cell morphology.

Main Results:

  • Scaffolds significantly increased hBMSC Z-Depth compared to the 2D planar substrate, indicating less flattened cells.
  • The 'Dimensionality Matrix' demonstrated that scaffold properties influenced hBMSC shape, driving them into 1D, 2D, or 3D configurations.
  • Results confirm that hBMSCs exhibit greater 3D character in scaffolds than on planar surfaces.

Conclusions:

  • hBMSCs adopt more three-dimensional morphologies within scaffolds compared to 2D substrates.
  • Scaffold properties can be modulated to control and direct cell dimensionality.
  • The developed measurement approach aids in evaluating scaffold designs for optimal 3D cell niches.