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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis01:22

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

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Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
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Stem Cell Culture01:17

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Related Experiment Video

Updated: May 3, 2026

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines
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Spermatogonial stem cell functions in physiological and pathological conditions.

Qi-En Yang1, Jon M Oatley1

  • 1Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA.

Current Topics in Developmental Biology
|January 21, 2014
PubMed
Summary

Spermatogonial stem cells (SSCs) are crucial for sperm production and fertility. Understanding their development is key to preventing infertility and testicular germ cell tumors.

Keywords:
AzoospermiaSelf-renewalSertoli-cell-onlySpermatogonial stem cellsTesticular germ cell tumorTranscription factors

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Stem cell biology

Background:

  • Spermatogenesis is essential for species continuity, relying on spermatogonial stem cells (SSCs).
  • The initial formation of the SSC pool from embryonic gonocyte precursors during neonatal development is critical for adult fertility.
  • Disruptions in SSC establishment or self-renewal can lead to infertility and testicular germ cell tumors.

Purpose of the Study:

  • To investigate the largely undefined molecular mechanisms underlying the initial formation of the SSC pool.
  • To identify key regulators of SSC self-renewal and their role in spermatogenesis.
  • To explore the link between SSC dysregulation and reproductive health issues.

Main Methods:

  • Utilized mutant mouse models to study SSC function.
  • Employed experimental manipulation within primary cultures of mouse SSCs.
  • Analyzed transcription factors and posttranscriptional regulators involved in SSC self-renewal.

Main Results:

  • Identified several transcription factors and posttranscriptional regulators crucial for SSC self-renewal.
  • Demonstrated the importance of these factors in maintaining the spermatogenic lineage.
  • Provided insights into molecular mechanisms governing SSC pool establishment.

Conclusions:

  • The molecular mechanisms governing SSC pool formation are critical for male fertility.
  • Dysregulation of SSC self-renewal factors can cause infertility and potentially contribute to testicular germ cell tumors.
  • Further research into these regulators could lead to new therapeutic strategies for reproductive disorders.