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Updated: May 3, 2026
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
A procedure for the in vivo determination of enzyme activity in higher plant tissue
1Department of Botany, The University, M13 9PL, Manchester, UK.
Abstract:
Rapid freezing of higher plant tissue in liquid nitrogen renders the cells permeable to a wide range of substrate molecules. Tissue permeabilized by repeated freeze-thaw treatment can be used for the measurement of several enzymes. With most of the tissues examined maximum in vivo activities were obtained using a combination of freeze-thaw treatment followed by vacuum infiltration. The activities and properties of enzymes determined with this procedure are very comparable with those obtained for enzymes prepared by conventional extraction procedures.
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