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Updated: Aug 16, 2026

05:31
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
Published on: August 30, 2007
[Specific for apurine-apyrimidine DNA endodeoxyribonuclease from the rat brain]
Biokhimiia (Moscow, Russia)
|July 1, 1987
Abstract:
Endodeoxyribonuclease was detected in rat neocortex chromatin. The partly purified enzyme was found to influence the superhelical apurine-apyrimidine DNA 50 times as effectively as compared to the native substrate. The enzyme hydrolyzes the phosphodiester bond with the formation of 3'-OH- and 5'-phosphate terminal groups. The enzyme-hydrolyzed DNA is an effective primer for DNA-polymerase I from E. coli. It was assumed that DNAase from rat brain chromatin is an apurine-apyrimidine endonuclease II.
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