Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rational modulation of immune mechanisms synergizes the anti-tumor effects of targeted radiation therapy in pre-clinical models.

Frontiers in immunology·2026

Same author

Digital Pathology and Artificial Intelligence Applied to Nonclinical Toxicology Pathology-The Current State, Challenges, and Future Directions.

Toxicologic pathology·2025

Same author

FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma.

Nature communications·2025

Same author

A Novel B7-H4xCD3 Bispecific T-cell Engager (PF-07260437) Synergizes with Breast Cancer Standard of Care and Immune Checkpoint Therapies.

Molecular cancer therapeutics·2025

Same author

18F-FLT PET, a Noninvasive Pharmacodynamic Biomarker of Tumor Cell Proliferation, Detected Differential Response to Various Cyclin-Dependent Kinase (CDK) Inhibitors.

Molecular cancer therapeutics·2025

Same author

Differential effects of FcRn antagonists on the subcellular trafficking of FcRn and albumin.

JCI insight·2024

Same journal

LEARNABLE HIERARCHICAL VISUAL CONTEXTS FOR TUMOR SEGMENTATION IN COMPUTED TOMOGRAPHY IMAGES.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

Same journal

DUAL CROSS-ATTENTION SIAMESE TRANSFORMER FOR RECTAL TUMOR REGROWTH ASSESSMENT IN WATCH-AND-WAIT ENDOSCOPY.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

Same journal

LUMEN: LONGITUDINAL MULTI-MODAL RADIOLOGY MODEL FOR PROGNOSIS AND DIAGNOSIS.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

Same journal

OVERVIEW OF THE CXR-LT 2026 CHALLENGE: MULTI-CENTER LONG-TAILED AND ZERO SHOT CHEST X-RAY CLASSIFICATION.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

Same journal

CROSS-MODAL FINE-TUNING OF 3D CONVOLUTIONAL FOUNDATION MODELS FOR ADHD CLASSIFICATION WITH LOW-RANK ADAPTATION.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

Same journal

AN IN SILICO STUDY OF LOW-INTENSITY FOCUSED ULTRASOUND DISPLACEMENT MAPPING WITH A 220 KHZ CLINICAL PHASED-ARRAY TRANSDUCER.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 3, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

3D single molecule tracking and superresolution microscopy using multifocal plane microscopy.

Sripad Ram¹, E Sally Ward¹, Raimund J Ober²

¹Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Proceedings. IEEE International Symposium on Biomedical Imaging

|January 21, 2014

Summary

This summary is machine-generated.

Multifocal plane microscopy (MUM) offers advanced 3D imaging for tracking cellular events and visualizing sub-cellular structures. This technique overcomes key challenges in 3D single molecule localization for enhanced biological research.

Keywords:

2D/3D localization accuracy Cramer-Rao lower bound Fisher information matrix maximum likelihood estimator resolution limits

More Related Videos

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

Related Experiment Videos

Last Updated: May 3, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

Area of Science:

Cellular biology
Microscopy
Biophysics

Background:

3D cellular process studies are limited by current imaging techniques.
Fast 3D tracking and superresolution imaging are crucial for understanding sub-cellular structures.

Purpose of the Study:

Introduce multifocal plane microscopy (MUM) as a novel 3D imaging modality.
Address technical challenges in 3D single molecule localization.
Demonstrate MUM's capability for 3D tracking and superresolution microscopy.

Main Methods:

Development of multifocal plane microscopy (MUM).
Analysis of technical challenges in 3D single molecule localization.
Application of MUM for 3D single molecule tracking and superresolution imaging.

Main Results:

MUM provides a powerful approach for 3D imaging.
The technique overcomes limitations in 3D single molecule localization.
Enables fast 3D tracking of cellular events and 3D superresolution of sub-cellular structures.

Conclusions:

MUM significantly advances 3D cellular imaging capabilities.
Overcomes critical hurdles in 3D single molecule localization.
Paves the way for new discoveries in cellular dynamics and structure.