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Updated: May 3, 2026
Author Spotlight: Detection and Treatment of Helicobacter pylori Infection
Published on: July 28, 2023
Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
Hirendra Nath Banerjee1, Monique Gramby1, Zack Hawkins1
1Department of Biological Sciences, Elizabethcity State University under, The University of North Carolina, Elizabethcity, NC-27909, USA.
Aim:
Rapid detection of H.pylori strains by PCR-Sequencing.
Methods:
16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software.
Results:
The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H.pylori strain#26695.
Conclusion:
The pathogenicity of H.pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.
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