Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemoradiotherapy versus radiotherapy in patients with advanced nasopharyngeal cancer: phase III randomized Intergroup study 0099.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2023
Same author

A systematic review of active treatment options in patients with desmoid tumours.

Current oncology (Toronto, Ont.)·2014
Same author

Treatment and follow-up strategies in desmoid tumours: a practice guideline.

Current oncology (Toronto, Ont.)·2014
Same author

Enhanced therapeutic effect of amsalog (CI-921) in combination with cisplatin in vitro and in vivo.

Oncology reports·2011
Same author

Should we kill the case report?

Veterinary pathology·2008
Same author

Do u smoke after txt? Results of a randomised trial of smoking cessation using mobile phone text messaging.

Tobacco control·2005

Related Experiment Video

Updated: Mar 29, 2026

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

10.5K

Solid tumor preparation for flow cytometry using a standard murine model.

J F Ensley1, Z Maciorowski, H Pietraszkiewicz

  • 1Department of Internal Medicine, Wayne State University, Detroit, Michigan 48201.

Cytometry
|September 1, 1987
PubMed
Summary

Preparing solid tumors for flow cytometry (FCM) is challenging. A combination of trypsin and collagenase enzymes, followed by ethanol-based fixation, optimizes cell yield, viability, and DNA histogram quality for FCM analysis.

More Related Videos

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
08:09

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Published on: April 6, 2015

12.2K
Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
14:15

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

26.9K

Related Experiment Videos

Last Updated: Mar 29, 2026

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

10.5K
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
08:09

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Published on: April 6, 2015

12.2K
Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
14:15

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

26.9K

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry (FCM) is crucial for analyzing cellular DNA in solid tumors.
  • Preparing single-cell suspensions from solid tumors, especially squamous cell carcinomas (SCCs), is difficult due to high desmosomal content.
  • Existing FCM preparation methods yield low cell viability and altered cellular parameters.

Purpose of the Study:

  • To optimize techniques for preparing solid human tumors for flow cytometric analysis.
  • To identify effective dissociation and fixation methods for high-yield, viable single-cell suspensions.
  • To establish a reliable murine tumor model for testing FCM preparation protocols.

Main Methods:

  • Comparative testing of various dissociation techniques: mechanical, enucleation, chemical, single enzymes, and combined enzymes (trypsin and collagenase).
  • Evaluation of different fixation systems: glutaraldehyde, paraformaldehyde, acetic acid, and ethanol-water mixtures with fetal calf serum.
  • Assessment of cell yield, viability (dye exclusion), cell loss, and DNA histogram quality.

Main Results:

  • Combination enzyme treatment (trypsin and collagenase) yielded the highest cell numbers in the shortest time with superior viability and cost-effectiveness.
  • Ethanol-water fixation systems with fetal calf serum minimized cell loss and produced high-quality, stable DNA histograms.
  • A murine SCC model closely mimicked human tumor histology, proving effective for technique optimization.

Conclusions:

  • Optimized dissociation and fixation protocols significantly improve the preparation of solid tumors for FCM.
  • Combined enzyme treatment and ethanol-based fixation are recommended for reliable FCM analysis of solid tumors.
  • Murine models are valuable for developing and validating FCM preparation techniques for human tumor studies.