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Updated: May 3, 2026
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Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy
Published on: November 30, 2018
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Mechanisms of fibrinogen adsorption at solid substrates
Zbigniew Adamczyk, Anna Bratek-Skicki, Paulina Żeliszewska
1J. Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30- 239 Cracow, Poland. ncadamcz@cyf-kr.edu.pl.
Current Topics in Medicinal Chemistry
|January 22, 2014
Summary
This review analyzes fibrinogen adsorption on solid surfaces, focusing on electrostatic interactions. It defines adsorption mechanisms and molecule orientations for applications in immunological assays.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Protein Adsorption
Background:
- Fibrinogen adsorption influences biomaterial performance and diagnostic assays.
- Understanding adsorption mechanisms is crucial for controlling surface interactions.
Purpose of the Study:
- To critically review recent findings on fibrinogen adsorption at solid/electrolyte interfaces.
- To quantitatively analyze adsorption processes, emphasizing electrostatic interactions.
- To define probable adsorption mechanisms based on physicochemical data.
Main Methods:
- Analysis of fibrinogen's primary chemical structure and physicochemical properties.
- Hydrodynamic, dynamic light scattering, and micro-electrophoretic measurements.
- Theoretical modeling to define conformations and charge distribution.
- Adsorption kinetics studies on hydrophilic and hydrophobic substrates.
- Electrokinetic and concentration depletion methods for carrier particles.
- Analysis of colloid particle deposition on fibrinogen monolayers.
Main Results:
- Defined fibrinogen conformations and charge distribution across various pH and ionic strengths.
- Characterized adsorption kinetics on different substrates and carrier particles.
- Discussed adsorption reversibility, molecule orientations, and maximum coverages.
- Evaluated the stability of fibrinogen monolayers under varying conditions.
- Derived information on molecule orientations from colloid particle deposition.
Conclusions:
- Established probable adsorption mechanisms of fibrinogen on solid/electrolyte interfaces.
- Highlighted the importance of electrostatic interactions in fibrinogen adsorption.
- Provided insights into fibrinogen behavior relevant to immunological assays and biomaterial design.
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