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Characterization of mononuclear cell-derived histamine release enhancing factor
R E Younger1, H G Herrod, P L Lieberman
1Department of Pediatrics, University of Tennessee Center for the Health Sciences, Chattanooga 37403.
The Journal of Allergy and Clinical Immunology
|October 1, 1987
Summary
Histamine release enhancing factor (HREF) boosts histamine release from basophils. This unique cytokine may play a role in inflammatory and atopic conditions.
Area of Science:
- Immunology
- Cell Biology
- Cytokine Research
Background:
- Histamine release enhancing factor (HREF) is produced by stimulated mononuclear cells.
- HREF enhances immunoglobulin E (IgE)-mediated histamine release from basophils in vitro.
- Understanding HREF's role is crucial for inflammatory and atopic disease research.
Purpose of the Study:
- To characterize the properties and source of HREF.
- To investigate the factors influencing HREF production and activity.
- To differentiate HREF from known cytokines and mediators.
Main Methods:
- Culturing phytohemagglutinin-stimulated mononuclear cells.
- Assessing HREF activity by measuring basophil histamine release.
- Determining factor stability (56°C) and molecular weight (10-30 kDa).
Main Results:
- HREF production is concentration-dependent on mononuclear cells and the factor itself.
- HREF is stable at 56°C and has a molecular weight between 10,000-30,000 daltons.
- HREF activity was not due to phytohemagglutinin, interferons, arachidonic acid metabolites, or interleukins 1 or 2.
Conclusions:
- HREF is a distinct cytokine with significant potential in understanding inflammatory and atopic processes.
- HREF's unique characteristics suggest a novel pathway in immune responses.
- Further research into HREF could reveal new therapeutic targets for allergic diseases.
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