Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

22.9K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
22.9K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.8K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of haploinsufficiency in human neural progenitor cells: insights into early molecular effects of autism-related genes.

Cell death & disease·2026
Same author

SIRT7 regulates dosage compensation and safeguards the female X chromosome.

Nature·2026
Same author

Borne to be wild: ecDNA as a vehicle for oncogenic fusion.

Molecular cell·2026
Same author

A single-cell CRISPR screen defines a gene regulatory network governing human pluripotency in primed and naive cells.

Cell reports·2026
Same author

Chromosomal instability and chromosome 17p loss drive convergent <i>NDE1</i> synthetic lethality in metastatic cancer cells.

bioRxiv : the preprint server for biology·2026
Same author

Holding the Line: Negative Selection Maintains the Karyotype in Metastatic Colorectal Cancer.

Cancer discovery·2026

Related Experiment Video

Updated: May 3, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K

Quality control: Genome maintenance in pluripotent stem cells.

Uri Weissbein1, Nissim Benvenisty, Uri Ben-David

  • 1Stem Cell Unit, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.

The Journal of Cell Biology
|January 22, 2014
PubMed
Summary

Pluripotent stem cells (PSCs) require stable genomes for self-renewal and differentiation. This study explores methods to protect PSCs from genomic damage during in vitro culture, crucial for regenerative medicine applications.

More Related Videos

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

9.2K
Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility
06:11

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

2.2K

Related Experiment Videos

Last Updated: May 3, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

9.2K
Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility
06:11

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

2.2K

Area of Science:

  • Stem cell biology
  • Genomics
  • Cellular and molecular medicine

Background:

  • Pluripotent stem cells (PSCs) are vital for research and regenerative medicine.
  • Maintaining genomic integrity in PSCs is essential for their function.
  • In vitro culture conditions pose risks to PSC genome stability.

Purpose of the Study:

  • To investigate challenges to genome maintenance in PSCs during prolonged culture.
  • To identify strategies for alleviating genomic insults in PSCs.
  • To develop methods for early detection of genomic aberrations in PSCs.

Main Methods:

  • Review of recent research on PSC genome maintenance.
  • Analysis of environmental factors affecting PSC genomic stability.
  • Exploration of techniques for detecting genomic damage in PSCs.

Main Results:

  • Prolonged in vitro propagation and culture conditions threaten PSC genomic integrity.
  • Genomic aberrations can arise from standard PSC culture methods.
  • Early detection of genomic damage is critical for PSC applications.

Conclusions:

  • Protecting PSC genome stability is paramount for their therapeutic potential.
  • Developing methods to mitigate and detect genomic insults is a key research focus.
  • Ensuring genomic integrity will advance the use of PSCs in regenerative medicine and basic research.