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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Mass Spectrum: Interpretation01:24

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Chemical Ionization (CI) Mass Spectrometry01:21

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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Related Experiment Video

Updated: May 3, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

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Electrostatic spray ionization mass spectrometry imaging.

Liang Qiao1, Elena Tobolkina, Andreas Lesch

  • 1Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne , Station 6, CH-1015 Lausanne, Switzerland.

Analytical Chemistry
|January 23, 2014
PubMed
Summary
This summary is machine-generated.

A new mass spectrometry imaging method uses electrostatic spray ionization for ambient analysis. This technique provides quantitative data for diverse molecules, even with excess salts, enabling detailed imaging of samples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Mass spectrometry imaging (MSI) requires localized desorption/ionization and detection for spatial resolution.
  • Existing MSI methods face challenges with certain sample types and conditions.

Purpose of the Study:

  • To develop a novel, ambient mass spectrometry imaging method.
  • To enable quantitative analysis of diverse molecules under ambient conditions.

Main Methods:

  • Utilized electrostatic spray ionization for mass spectrometry imaging.
  • Applied the method to various samples including protein/peptide spots, dyes, and cells.

Main Results:

  • Achieved 2D mass spectrometry images of protein and peptide spots, inkjet-printed black dye patterns, and cells.
  • Demonstrated quantitative analysis capability, even in the presence of excess salts.
  • Validated the method's applicability to a wide range of molecules.

Conclusions:

  • The novel electrostatic spray ionization-based MSI method operates under ambient conditions.
  • This technique offers broad applicability in analytical and bioanalytical chemistry for high-resolution imaging.