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Plasma antibody profiles in non-human primate tuberculosis
Resmi Ravindran1, Viswanathan V Krishnan, Rajeev Dhawan
1Center for Comparative Medicine, University of California, Davis, CA, USA.
Journal of Medical Primatology
|January 23, 2014
Summary
A new multiplex assay identifies antibodies against Mycobacterium tuberculosis in non-human primates (NHPs). This tool offers a high-throughput alternative for tuberculosis screening in NHPs, improving upon traditional methods.
Area of Science:
- Veterinary immunology
- Infectious disease diagnostics
- Primate research
Background:
- Tuberculosis (TB) in non-human primates (NHPs) is a significant zoonotic concern.
- Current diagnostic methods like the tuberculin skin test lack sufficient sensitivity and specificity.
- Efficient identification of Mycobacterium tuberculosis infected NHPs is crucial for disease control.
Purpose of the Study:
- To develop a novel immunoassay for detecting plasma antibodies against M. tuberculosis in NHPs.
- To address the challenge of variable antigen recognition among infected animals.
- To create a multiplex panel for comprehensive antibody detection.
Main Methods:
- Development of a multiplex panel utilizing the Luminex® platform.
- Testing the panel against 28 M. tuberculosis antigens.
- Analysis of antibody responses in experimentally and naturally infected NHPs (rhesus and cynomolgus macaques).
Main Results:
- Antibodies against eight specific antigens were detected in experimentally infected NHPs.
- Naturally infected rhesus macaques with lung TB pathology showed antibodies to five additional antigens.
- A total of 13 M. tuberculosis antigens were identified as key targets.
Conclusions:
- The developed multiplex panel is user-friendly and easily implementable.
- This assay offers a high-throughput alternative for NHP TB screening.
- The panel enhances diagnostic capabilities for Mycobacterium tuberculosis in non-human primates.
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