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Updated: May 3, 2026

Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
Published on: April 28, 2023
CYB5A and autophagy-mediated cell death in pancreatic cancer
Elisa Giovannetti1, Giuseppe Giaccone2
1Department of Medical Oncology; VU University Medical Center; Amsterdam, The Netherlands; Start-Up Unit; University of Pisa; Pisa, Italy.
Abstract:
The highly invasive and chemoresistant phenotype of pancreatic cancer highlights the urgency to identify prognostic biomarkers and novel therapeutic targets. Recently, we observed a significant correlation between shorter survival and loss of the cytoband 18q22.3. Here we investigated genes encoded by this cytoband, and demonstrated the prognostic value of CYB5A in resected and metastatic patients. Furthermore, our in vitro and in vivo studies clarified CYB5A inhibitory activity of oncogenic phenotypes through autophagy induction. This raises the possibility that inhibition of CYB5A-deregulated downstream pathways, such as those involving TRAF6, may favor autophagy-mediated cancer cell death in selected subgroups of patients.
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