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Related Experiment Video

Updated: May 3, 2026

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
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Universal marker and detection tool for human sarcoma circulating tumor cells.

Arun Satelli1, Abhisek Mitra, Jeffry J Cutrera

  • 1Authors' Affiliations: Departments of Pediatrics and Surgical Oncology; Department of Sarcoma Medical Oncology, The University of Texas MD Anderson Cancer Center; and UTMD Graduate School of Biomedical Sciences, Houston, Texas.

Cancer Research
|January 23, 2014
PubMed
Summary

Researchers identified cell-surface vimentin (CSV) as a universal marker for detecting circulating tumor cells (CTCs) in sarcoma patients. This discovery offers a new prognostic tool for monitoring cancer metastasis and relapse.

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Area of Science:

  • Oncology
  • Biomarker Discovery
  • Cancer Diagnostics

Background:

  • No specific marker currently exists for detecting circulating tumor cells (CTCs) across diverse sarcoma types.
  • Existing CTC detection tools are primarily designed for epithelial cancers, not sarcomas.

Purpose of the Study:

  • To identify a universal and specific cell-surface marker for sarcoma CTCs.
  • To develop a novel diagnostic tool for monitoring sarcoma metastasis and relapse.

Main Methods:

  • Development of a novel monoclonal antibody targeting cell-surface vimentin (CSV).
  • Isolation and enumeration of sarcoma CTCs from patient blood using CSV as a probe.
  • Validation of CTC phenotype via single-cell genomic amplification, mutation detection, and FISH.

Main Results:

  • Cell-surface vimentin (CSV) identified as an exclusive marker on sarcoma CTCs, irrespective of tumor origin.
  • High sensitivity and specificity achieved in isolating and enumerating sarcoma CTCs.
  • Successful phenotypic validation of isolated CTCs.

Conclusions:

  • Cell-surface vimentin (CSV) is established as the first universal and specific CTC marker for sarcomas.
  • This marker provides a crucial prognostic tool for tracking cancer spread and recurrence.
  • The findings pave the way for improved monitoring of sarcoma patients.