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Streptavidin binding as a model to characterize thiol-ene chemistry-based polyamine surfaces for reversible photonic
Eva Melnik1, Paul Muellner, Ole Bethge
1AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220 Vienna, Austria. Eva.Melnik@ait.ac.at.
Abstract:
Biotin- and iminobiotin-bonded surfaces obtained by thiol-ene chemistry and subsequent modification with polyamines were characterized with respect to streptavidin-binding capacity and reversibility for photonic biosensing using X-ray photoelectron spectroscopy and Mach-Zehnder-interferometric sensors. The streptavidin-iminobiotin system was exploited for reversible multilayer deposition and determination of affinity constants on each layer.

