Related Experiment Video
Updated: May 3, 2026
Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
Published on: October 20, 2023
Influence of pectins on NADPH oxidase and phagocytic activity of neutrophils during cryopreservation
O O Zaitseva1, T V Polezhaeva2, A N Khudyakov2
1Laboratory of Cryophysiology of Blood (T.V. Polezhaeva) of the Physiology Institute at the Komi Scientific Center, Ural Branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russian Federation. ddic@yandex.ru
Abstract:
NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.
More Related Videos
06:42Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
Published on: September 1, 2020
13:35Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Published on: May 5, 2017