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Related Experiment Video

Updated: May 3, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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Acoustic focusing with engineered node locations for high-performance microfluidic particle separation.

Erika J Fong1, Amanda C Johnston, Timothy Notton

  • 1Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA, 94550 USA. shusteff1@llnl.gov.

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|January 23, 2014
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Summary
This summary is machine-generated.

This study introduces novel acoustofluidic devices with a transparent wall design, enabling precise control over particle focusing locations within microchannels for advanced biological sample processing.

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Area of Science:

  • Microfluidics
  • Acoustofluidics
  • Biotechnology

Background:

  • Acoustofluidic devices offer gentle, high-speed manipulation of microparticles for biological sample processing.
  • Conventional devices are limited to focusing particles at fixed fractional locations within the channel width.

Purpose of the Study:

  • To develop a novel acoustophoretic device design overcoming limitations of particle focusing locations.
  • To enable arbitrary particle focusing anywhere within a microchannel.

Main Methods:

  • Fabrication of a parallel fluid channel separated by a thin silicon wall from the sample channel.
  • Utilizing coupled ultrasound resonance in both channels to de-couple fluidic and acoustic boundaries.
  • Investigating device configurations to optimize particle focusing and separation efficiency.

Main Results:

  • Demonstrated that a thin wall negligibly impacts focusing efficiency and location.
  • Achieved high-efficiency focusing of 10 μm microspheres (>90% extraction at 450 μL/min).
  • Successfully separated cell-free viruses from mammalian cells (98% cell purity, 70% virus purity at 100 μL/min).

Conclusions:

  • The transparent wall acoustofluidic device design allows precise, arbitrary particle focusing.
  • This approach maintains high separation performance, enabling applications like virus-cell separation.