Enantioselective surface plasmon resonance sensor based on C60 fullerene-glutathione self-assembled monolayer (SAM)

A novel enantioselective surface plasmon resonance (SPR) sensor based on a self-assembled monolayer of C60 fullerene as the chiral selector is proposed. A binding assay, apparent affinity constant, and apparent dissociation binding constant were used to analyze and study the enantioselectivity of C60 fullerene-glutathione film for L-histidine, which was chosen as the model analyte. The apparent affinity constant for the complex formed by L-histidine with C60 fullerene-glutathione film was 5.2 x 10(9)  M(-1). The proposed SPR sensor can be used for the assay of L-histidine in the 10(-10)-10(-7)  mol/L concentration range.