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08:15
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
Published on: September 28, 2018
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High-throughput miniaturized bioreactors for cell culture process development: reproducibility, scalability, and
Shahid Rameez1, Sigma S Mostafa, Christopher Miller
1Process Development and Manufacturing, KBI Biopharma Inc., 2 Triangle Dr, Research Triangle Park, NC, 27709.
Biotechnology Progress
|January 23, 2014
Summary
The Advanced Microscale Bioreactors (ambr™) system accelerates biopharmaceutical development by accurately mimicking large-scale bioreactor performance. This high-throughput platform enables reliable cell culture process development and optimization.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Cell Culture Technology
Background:
- Accelerating biopharmaceutical development is crucial.
- Traditional bioreactor development is time-consuming.
- Advanced Microscale Bioreactors (ambr™) offer a potential solution.
Purpose of the Study:
- To evaluate the performance of the ambr™ system for monoclonal antibody production.
- To compare ambr™ performance against conventional bioreactor systems.
- To assess the scalability and process control capabilities of ambr™.
Main Methods:
- Utilized ambr™ automated micro-bioreactor system (10-15 mL working volume).
- Produced monoclonal antibody using a recombinant Chinese Hamster Ovary (CHO) cell line.
- Compared ambr™ results with 3 L, 15 L, and 200 L stirred tank bioreactors.
- Introduced complex feed formulations, perturbations, and controlled critical process parameters (temperature, DO, pH).
Main Results:
- ambr™ demonstrated comparable cell culture profiles across scales.
- Process parameter changes in ambr™ predicted cell growth, viability, and titer accurately.
- The system successfully reproduced variations in temperature, dissolved oxygen (DO), and pH.
- ambr™ responded effectively to perturbations in pH and DO via control loop adjustments.
Conclusions:
- The ambr™ system is a valuable high-throughput tool for cell culture process development.
- It accurately replicates large-scale bioreactor conditions and responses.
- ambr™ facilitates accelerated and reliable biopharmaceutical development.
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