[DNA, RNA and protein synthesis in hepatitis A virus-infected cells]

Synthesis of DNA, RNA, and protein macromolecules was observed in cells infected with a strain of HA virus which had undergone 11 passages in the same culture. At 24-hour intervals the infected and uninfected cells were pulse-labeled with 1 microCi/cc 3H-thymidine, 3H-uridine, and 14C-valine. In 6-9 days after cell inoculation, inhibition of DNA, RNA, and protein synthesis was observed, being most marked in DNA synthesis. The synthesis of these macromolecules increases on the 10-13th day which coincides with virus antigen accumulation in the cell cytoplasm.