Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

705
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
705
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
Biodiversity and Human Values01:24

Biodiversity and Human Values

14.8K
Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather patterns and therefore human civilizations.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Multifrequency Electromagnetic Pulses on tumor growth in immunocompetent mice.

Scientific reports·2026
Same author

Pruning the Tree: Comparing OTUs and ASVs in High-Throughput Sequencing of 5S-IGS Nuclear Ribosomal DNA in Phylogenetic Studies.

Ecology and evolution·2025
Same author

Authentication of the Botanical Origin of Honey: <i>In Silico</i> Assessment of Primers for DNA Metabarcoding.

Journal of agricultural and food chemistry·2025
Same author

Assessment of molecular modulation by multifrequency electromagnetic pulses to preferably eradicate tumorigenic cells.

Scientific reports·2024
Same author

Taxonomic and metabolic characterisation of biofilms colonising Roman stuccoes at Baia's thermal baths and restoration strategies.

Scientific reports·2024
Same author

Comparative analysis of the microbial plastisphere at three sites along the Sarno river (Italy).

Environmental pollution (Barking, Essex : 1987)·2024

Related Experiment Video

Updated: May 3, 2026

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

2.3K

DNA barcoding as a complementary tool for conservation and valorisation of forest resources.

Angeliki Laiou1, Luca Aconiti Mandolini1, Roberta Piredda1

  • 1Department of Agriculture, Forests, Nature and Energy (DAFNE) - Università degli Studi della Tuscia, via S. Camillo de' Lellis, 01100 Viterbo, Italy.

Zookeys
|January 24, 2014
PubMed
Summary

DNA barcoding shows limitations for forest surveys, with 66.7% species identification success. Improvements in technical protocols and public databases are needed for effective forest tree identification and conservation.

Keywords:
ConservationDNA barcodingForest BiodiversityMedicinal and Aromatic plants

More Related Videos

Field Identification of Matricaria chamomilla using a Portable qPCR System
12:08

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

7.0K
Collecting and Processing Drone-based Remotely Sensed Data for Use in Forest Recovery Monitoring
08:16

Collecting and Processing Drone-based Remotely Sensed Data for Use in Forest Recovery Monitoring

Published on: October 24, 2025

918

Related Experiment Videos

Last Updated: May 3, 2026

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

2.3K
Field Identification of Matricaria chamomilla using a Portable qPCR System
12:08

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

7.0K
Collecting and Processing Drone-based Remotely Sensed Data for Use in Forest Recovery Monitoring
08:16

Collecting and Processing Drone-based Remotely Sensed Data for Use in Forest Recovery Monitoring

Published on: October 24, 2025

918

Area of Science:

  • Botany
  • Molecular Biology
  • Conservation Science

Background:

  • Forests are vital global resources for healthcare and economy, providing botanical raw materials.
  • DNA barcoding is a molecular technique for rapid species identification and biodiversity assessment.
  • Accurate identification of forest tree species is crucial for their conservation and trade control.

Purpose of the Study:

  • To test the in situ application of DNA barcodes for identifying forest tree species.
  • To evaluate the universality, data retrieval, and species assignment accuracy of core DNA barcode markers.
  • To assess limitations in DNA barcoding for taxonomic forest surveys.

Main Methods:

  • Tested the "core barcode" markers (rbcL, matK, trnH-psbA) on 68 forest tree specimens from 24 taxa.
  • Evaluated amplification and sequencing success rates for each marker.
  • Assessed species identification success, DNA barcoding gaps, and GenBank discrimination.

Main Results:

  • RbcL and trnH-psbA showed 100% amplification and sequencing success; matK had lower success.
  • The trnH-psbA region exhibited high genetic variability but often lacked a clear DNA barcoding gap.
  • Overall species identification success was 66.7%, limited by marker performance and incomplete public databases.

Conclusions:

  • Current DNA barcoding protocols have limitations for comprehensive forest tree surveys.
  • Improvements in technical methods and expansion of public reference databases are necessary.
  • Enhanced DNA barcoding application can aid in forest resource management and conservation efforts.