Related Experiment Video
Updated: May 3, 2026
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Effects of simultaneous nicotine and alcohol use in periodontitis progression in rats: A histomorphometric study
Daniel F Pereira Vasconcelos1, Marco A Dias da Silva2, Marcelo Rocha Marques3
1Division of Histology and Embryology, School of Biomedicine, Federal University of Piauí, Parnaíba-PI, Brazi.
Objective:
The aim of this study was to evaluate the effects of alcohol and nicotine, when used alone or simultaneously, on the alveolar bone loss area resulting from ligature-induced periodontitis in rats.
Study Design:
Forty adult male rats received a cotton ligature in the first lower molar sulcular area, and the animals were randomly assigned to different treatments (n = 10, each group) including daily peritoneal injections of saline solution (group A), submitted to self-administration of alcohol 25% (group B), nicotine solution in concentration 0.19 μl/ml (group C), and nicotine solution in concentration 0.19 μl/ml plus self-administration of alcohol 25% (group D). Five weeks later, the animals were sacrificed, and the samples were routinely processed for semi-serial decalcified sections.
Results:
Ligated teeth showed more alveolar bone loss than unligated ones (p < 0.05). Unligated teeth showed no significant differences between each other (p > 0.05). Analyses between the ligated teeth showed that the group C (nicotine) or group B (alcohol 25%) each had increasing alveolar bone loss in the furcation area, and the simultaneous combination alcohol and nicotine (group D) intensified these effects (p < 0.05).
Conclusion:
The results suggest that the simultaneous combination of alcohol and nicotine have a synergistic effect in the progression of periodontitis, evidenced by increased furcation region bone destruction in periodontal disease in rats. Key words:Alveolar bone loss, periodontitis, nicotine, alcohol, rats.
