Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

22.9K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
22.9K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.8K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

3.4K
3.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Naïve human iPSCs obtained by culturing the ICGi022-A cell line with primed pluripotency in HENSM medium efficiently differentiate into endothelial derivatives.

Vavilovskii zhurnal genetiki i selektsii·2026
Same author

Generation of the ICGi019-B-1 and ICGi019-B-2 lines via correction of the p.Met659Ile (c.1977G>A) variant in MYH7 of patient-specific induced pluripotent stem cells using CRISPR/Cas9.

Vavilovskii zhurnal genetiki i selektsii·2025
Same author

Isogenic induced pluripotent stem cell line ICGi036-A-1 from a patient with familial hypercholesterolaemia, derived by correcting a pathogenic variant of the gene LDLR c.530C>T.

Vavilovskii zhurnal genetiki i selektsii·2025
Same author

Generation and characterisation of seven induced pluripotent stem cell lines from two patients with Parkinson's disease carrying the pathological variant c.1087G>T of the LGR4 gene.

Vavilovskii zhurnal genetiki i selektsii·2025
Same author

Generation and characterization of two induced pluripotent stem cell lines (ICGi052-A and ICGi052-B) from a patient with frontotemporal dementia with parkinsonism-17 associated with the pathological variant c.2013T>G in the MAPT gene.

Vavilovskii zhurnal genetiki i selektsii·2024
Same author

PARP1 Gene Knockout Suppresses Expression of DNA Base Excision Repair Genes.

Doklady. Biochemistry and biophysics·2023

Related Experiment Video

Updated: May 3, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

10.8K

"Epigenetic memory" phenomenon in induced pluripotent stem cells.

E A Vaskova1, A E Stekleneva1, S P Medvedev1

  • 11Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, prosp. Akad. Lavrentyeva, 10, Novosibirsk, Russia, 630090 ; Meshalkin State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, Novosibirsk, Russia, 630055 ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, prosp. Akad. Lavrentyeva, 8, Novosibirsk, Russia, 630090.

Acta Naturae
|January 24, 2014
PubMed
Summary

Induced pluripotent stem cells (iPSCs) offer promising biomedical models. However, "epigenetic memory" from donor cells can influence iPSC properties, impacting their application in cell technologies.

Keywords:
epigeneticspluripotencyreprogramming

More Related Videos

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K
Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

4.7K

Related Experiment Videos

Last Updated: May 3, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

10.8K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K
Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

4.7K

Area of Science:

  • Biomedicine and pharmacology
  • Stem cell biology
  • Epigenetics

Background:

  • Induced pluripotent stem cells (iPSCs) are crucial for developing advanced biomedical and pharmacological models.
  • High-throughput molecular, epigenetic, and functional analyses are essential for iPSC applications.
  • While iPSCs resemble embryonic stem cells, they retain specific features from their somatic origin.

Purpose of the Study:

  • To review the phenomenon of "epigenetic memory" in induced pluripotent stem cells (iPSCs).
  • To discuss the impact of epigenetic memory on iPSC characteristics.
  • To explore the potential applications of epigenetic memory in cell technologies.

Main Methods:

  • Review of existing literature on iPSC reprogramming and epigenetic analysis.
  • Analysis of gene and microRNA expression patterns.
  • Assessment of DNA methylation and histone modification patterns.

Main Results:

  • iPSCs exhibit molecular and epigenetic profiles similar to embryonic stem cells.
  • Residual epigenetic signatures from donor somatic cells, termed "epigenetic memory," are present in iPSCs.
  • Epigenetic memory can influence iPSC properties and functionality.

Conclusions:

  • Understanding and managing epigenetic memory is critical for optimizing iPSC applications.
  • Epigenetic memory may offer novel avenues for cell technology development.
  • Further research is needed to fully elucidate the implications of epigenetic memory in iPSCs.