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LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
Stěpán Horník1, Marie Sajfrtová1, Jindřich Karban1
1Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Abstract:
The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.
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