Search research articles

Search research articles

Related Experiment Video

Updated: May 3, 2026

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Published on: November 22, 2015

Rapid enzymatic method for pectin methyl esters determination.

Lucyna Lękawska-Andrinopoulou¹, Efstathios G Vasiliou¹, Dimitrios G Georgakopoulos²

¹Chemistry, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

Journal of Analytical Methods in Chemistry

|January 24, 2014

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modulation of Egg Elemental Metabolomics by Dietary Supplementation with Flavonoids and Orange Pulp (<i>Citrus sinensis</i>).

Antioxidants (Basel, Switzerland)·2025

Same author

Wood Waste Valorization and Classification Approaches: A systematic review.

Open research Europe·2025

Same author

Elemental Fingerprinting of Pecorino Romano and Pecorino Sardo PDO: Characterization, Authentication and Nutritional Value.

Molecules (Basel, Switzerland)·2024

Same author

Industrial symbiosis platforms for synergy identification and their most important data points: a systematic review.

Open research Europe·2023

Same author

Compositional Differences of Greek Cheeses of Limited Production.

Foods (Basel, Switzerland)·2023

Same author

Olive Oil Benefits from Sesame Oil Blending While Extra Virgin Olive Oil Resists Oxidation during Deep Frying.

Molecules (Basel, Switzerland)·2023

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

A new automated flow method accurately determines pectin degree of methylation, crucial for food and pharma applications. This rapid, sustainable technique offers reliable results comparable to manual methods.

Area of Science:

Analytical Chemistry
Food Chemistry
Biochemistry

Background:

Pectin, a natural polysaccharide, is widely used in the food and pharmaceutical industries.
The degree of methylation in pectin is a critical parameter influencing its functional properties and applications.
Existing methods for determining pectin methyl esters can be time-consuming and labor-intensive.

Purpose of the Study:

To develop a rapid, fully automated, kinetic flow method for the precise determination of pectin methyl esters.
To establish a sustainable and efficient analytical approach for pectin characterization.
To introduce the first application of flow analysis for pectin methyl ester determination.

Main Methods:

A custom-built analyzer utilizing reverse flow-injection and stopped-flow principles was employed.

More Related Videos

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Published on: April 15, 2013

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Published on: February 4, 2022

Related Experiment Videos

Last Updated: May 3, 2026

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Published on: November 22, 2015

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Published on: April 15, 2013

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Published on: February 4, 2022

Pectin methyl ester was enzymatically hydrolyzed to release methanol.

Methanol was quantified via a coupled enzymatic reaction producing a colored product, monitored spectrophotometrically.

The system was controlled by a LabVIEW program for full automation.

Main Results:

The developed method achieved a detection limit of 1.47 mM with an analysis rate of 7 samples per hour.
Statistical analysis (paired t-test) confirmed the automated method's results are equivalent to a manual method at the 95% confidence interval.
The method demonstrated high precision and accuracy for pectin methyl ester determination.

Conclusions:

The novel automated flow method provides a rapid, reliable, and sustainable approach for determining pectin degree of methylation.
This technique represents a significant advancement in pectin analysis, offering advantages over traditional manual methods.
The successful implementation of flow analysis opens new avenues for efficient pectin characterization in industrial settings.