Bioactive phthalides from Ligusticum sinense Oliv cv. Chaxiong
Qian Wei1, Jianbo Yang1, Jin Ren1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Fitoterapia
|January 25, 2014
Summary
Researchers isolated novel phthalides from Ligusticum sinense, with some compounds showing neuroprotective effects. Certain isolates demonstrated higher cell survival in oxygen-glucose deprivation models than Ginsenoside Rg1.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Neuroscience
Background:
- Ligusticum sinense cv. Chaxiong is a traditional medicinal herb.
- Phthalides are a class of natural products with diverse biological activities.
Purpose of the Study:
- To isolate and characterize new and known phthalides from Ligusticum sinense cv. Chaxiong.
- To evaluate the neuroprotective potential of isolated compounds against oxygen-glucose deprivation in SK-N-SH cells.
Main Methods:
- Isolation and purification of compounds using chromatographic techniques.
- Structure elucidation using High-Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), UV, IR, and various Nuclear Magnetic Resonance (NMR) methods (1D and 2D).
- Determination of absolute configurations via Circular Dichroism (CD) spectroscopy and modified Mosher's method.
- In vitro cell-based assay for neuroprotection using SK-N-SH cells subjected to oxygen-glucose deprivation.
Main Results:
- Five new phthalides (1-4, 6), two new natural products (5, 7), and five known phthalides (8-12) were successfully isolated.
- The structures and absolute configurations of all isolated compounds were rigorously determined.
- Compounds 1-8 exhibited varying degrees of cell survival enhancement under oxygen-glucose deprivation.
- Compounds 1, 4, and 8 demonstrated superior neuroprotective effects compared to Ginsenoside Rg1 at a concentration of 10 μM.
Conclusions:
- The study successfully identified novel and known phthalides from Ligusticum sinense cv. Chaxiong.
- Several isolated phthalides, particularly compounds 1, 4, and 8, possess significant neuroprotective properties.
- These findings suggest potential therapeutic applications for these phthalides in neurological disorders.
