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Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain.

Bon Geun Goo1, You Jin Hwang2, Jae Kweon Park2

  • 1Department of Pharmaceutical Science, Gachon University, 191 Hambakmoye-ro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea.

Carbohydrate Research
|January 25, 2014
PubMed
Summary

A novel Bacillus strain, B. thuringiensis GU-2, was identified for producing high-purity gamma-cyclodextrin (γ-CD) under anaerobic conditions. This discovery advances anaerobic microbial production of γ-CD using gamma-cyclodextrin glycotransferase (γ-CGTase).

Keywords:
Bacillus thuringiensisCyclodextrinCyclodextrin glycotransferase (CGTase)γ-CD

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Area of Science:

  • Microbiology
  • Biochemistry
  • Enzyme Technology

Background:

  • Cyclodextrins (CDs) are cyclic oligosaccharides with diverse applications.
  • Gamma-cyclodextrin (γ-CD) production is typically achieved under aerobic conditions.
  • Efficient and specific production of γ-CD remains a challenge.

Purpose of the Study:

  • To isolate and characterize an anaerobic microbial strain capable of specific γ-CD production.
  • To optimize conditions for bacterial growth and γ-CD synthesis.
  • To identify the enzyme responsible for γ-CD production under anaerobic conditions.

Main Methods:

  • Isolation of anaerobic microbial strains from soil.
  • Cultivation in alkaline medium with starch substrate.
  • Optimization of pH, temperature, and substrate concentration.
  • Enzyme activity assays for gamma-cyclodextrin glycotransferase (γ-CGTase).

Main Results:

  • Isolation of Bacillus thuringiensis GU-2, an anaerobic γ-CD producer.
  • Optimal growth and γ-CD production at pH 8.5 and 37°C with 1.0% starch.
  • Achieved >95% purity of γ-CD from total CD yield.
  • Maximum γ-CGTase activity of 2.45 U/mL under optimized conditions.

Conclusions:

  • Bacillus thuringiensis GU-2 is the first reported anaerobic strain for specific γ-CD production.
  • The study demonstrates the feasibility of anaerobic γ-CD generation via γ-CGTase.
  • This finding opens new avenues for sustainable and efficient cyclodextrin production.