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Corneal storage in MK medium and K-Sol. Effect on ionic and non-ionic fluxes
D S Hull1, R Berdecia, K Green
1Department of Ophthalmology, Medical College of Georgia, Augusta 30912.
Abstract:
Rabbit corneas were stored at 4 degrees C for 3, 7 or 14 days in either modified MK medium or K-Sol. Corneal endothelial permeability to inulin following storage in modified MK was significantly less at each time examined than that found in corneas stored for either 3, 7 or 14 days in K-Sol. Inulin permeability after storage in K-Sol was increased at all times relative to unstored control corneal tissue, but only at 7 and 14 days in MK medium. Dextran permeability was similar following 3 days of storage in either solution, but dextran permeability following storage in modified MK was significantly less than the values found in corneas stored for 7 and 14 days in K-Sol. Dextran permeability was not significantly increased relative to control, at any storage time in MK medium but was increased at 7 and 14 days in K-Sol. Inulin and dextran permeabilities after storage in MK medium were maintained more closely to values found in fresh tissue than corneas stored in K-Sol. Net endothelial sodium fluxes following storage in modified MK medium were markedly less than those found in corneas stored for 3, 7 and 14 days in K-Sol. Net sodium fluxes are maintained better in K-Sol than in MK medium relative to control values. Net bicarbonate fluxes following storage in modified MK medium were significantly less than the 3-day values in K-Sol, but similar to the values after 7 and 14 days of K-Sol storage.(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
Modified MK medium better preserves corneal endothelial permeability and sodium flux compared to K-Sol after cold storage. This suggests MK medium may be superior for maintaining corneal tissue viability during storage.
Area of Science:
- Ophthalmology
- Tissue Engineering
- Biomaterials
Background:
- Corneal storage solutions are crucial for preserving donor tissue viability.
- Modified MK medium and K-Sol are commonly used storage solutions.
- Understanding their comparative efficacy is vital for improving corneal transplantation outcomes.
Purpose of the Study:
- To compare the efficacy of modified MK medium versus K-Sol in preserving rabbit corneal endothelial function after cold storage.
- To assess the impact of storage duration (3, 7, and 14 days) on corneal permeability and ion transport.
Main Methods:
- Rabbit corneas were stored at 4°C for 3, 7, or 14 days in either modified MK medium or K-Sol.
- Endothelial permeability to inulin and dextran was measured.
- Net endothelial sodium and bicarbonate fluxes were quantified.
Main Results:
- Modified MK medium significantly reduced inulin and dextran permeability compared to K-Sol at extended storage times.
- MK medium better maintained inulin and dextran permeability closer to fresh tissue levels.
- Net endothelial sodium fluxes were lower in MK medium, indicating better preservation, while K-Sol better maintained sodium fluxes relative to controls.
Conclusions:
- Modified MK medium demonstrates superior preservation of corneal endothelial permeability compared to K-Sol.
- MK medium appears more effective in maintaining corneal endothelial barrier function during extended cold storage.
- Further research may explore MK medium's potential for improving corneal graft survival.