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Related Experiment Videos

Streptococcus pyogenes type 12 M protein gene regulation by upstream sequences.

J C Robbins1, J G Spanier, S J Jones

  • 1Department of Microbiology, University of Minnesota, Minneapolis 55455.

Journal of Bacteriology
|December 1, 1987
PubMed
Summary

Group A Streptococcus M12 gene transcription is regulated by upstream DNA and multiple promoters. This study details the M12 gene sequence and its regulatory elements, crucial for understanding M protein expression.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Group A Streptococcus (GAS) produces M protein, a key virulence factor.
  • The emm12 gene encodes the Type 12 M protein, important for GAS pathogenesis.
  • Understanding M protein gene regulation is vital for developing targeted therapies.

Purpose of the Study:

  • To determine the nucleotide sequence of the emm12 gene and adjacent upstream DNA in GAS strain CS24.
  • To identify regulatory elements controlling M12 gene transcription.
  • To elucidate the transcriptional unit and structural features of the emm12 gene.

Main Methods:

  • Partial nucleotide sequencing of the emm12 gene and upstream DNA.
  • Analysis of M protein-specific mRNA transcription start sites.

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  • Deletion analysis of M protein-expressing plasmids.
  • DNA sequence analysis to identify repeat regions.

    • Main Results:

    • A 1,693 base pair sequence of the emm12 gene and upstream DNA was determined.
    • Transcription initiates from two promoters (P1 and P3).
    • An upstream region exceeding 1 kilobase is necessary for M-protein gene expression.
    • The emm12 gene is monocistronic and contains three major repeat regions (A, B, and C).

    Conclusions:

    • The emm12 gene has complex transcriptional regulation involving upstream DNA and multiple promoters.
    • Specific repeat regions within the emm12 gene contribute to its structure and regulation.
    • Findings provide insights into the molecular basis of M protein expression in Group A Streptococcus.