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Streptococcus pyogenes type 12 M protein gene regulation by upstream sequences
J C Robbins1, J G Spanier, S J Jones
1Department of Microbiology, University of Minnesota, Minneapolis 55455.
Journal of Bacteriology
|December 1, 1987
Summary
Group A Streptococcus M12 gene transcription is regulated by upstream DNA and multiple promoters. This study details the M12 gene sequence and its regulatory elements, crucial for understanding M protein expression.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Group A Streptococcus (GAS) produces M protein, a key virulence factor.
- The emm12 gene encodes the Type 12 M protein, important for GAS pathogenesis.
- Understanding M protein gene regulation is vital for developing targeted therapies.
Purpose of the Study:
- To determine the nucleotide sequence of the emm12 gene and adjacent upstream DNA in GAS strain CS24.
- To identify regulatory elements controlling M12 gene transcription.
- To elucidate the transcriptional unit and structural features of the emm12 gene.
Main Methods:
- Partial nucleotide sequencing of the emm12 gene and upstream DNA.
- Analysis of M protein-specific mRNA transcription start sites.
- Deletion analysis of M protein-expressing plasmids.
- DNA sequence analysis to identify repeat regions.
Main Results:
- A 1,693 base pair sequence of the emm12 gene and upstream DNA was determined.
- Transcription initiates from two promoters (P1 and P3).
- An upstream region exceeding 1 kilobase is necessary for M-protein gene expression.
- The emm12 gene is monocistronic and contains three major repeat regions (A, B, and C).
Conclusions:
- The emm12 gene has complex transcriptional regulation involving upstream DNA and multiple promoters.
- Specific repeat regions within the emm12 gene contribute to its structure and regulation.
- Findings provide insights into the molecular basis of M protein expression in Group A Streptococcus.