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Lipopolysaccharide impaired the functional activity of endothelial colony-forming cells
Min Yu1, Qingying Zhang, Qian Liu
1Department of Cardiology, First Affiliated Hospital, Shantou University Medical College, Shantou, PR China.
Background:
Recent studies have shown that endothelial progenitor cells (EPCs) contribute to lung repair after lipopolysaccharide (LPS)-induced lung injury and infusion of LPS decreased early EPCs in human peripheral blood. However, the effects of LPS on endothelial colony-forming cells (ECFCs) remain to be determined.
Objective:
To investigate possible effects of LPS on the functional activity of ECFCs.
Methods:
ECFCs were isolated from human umbilical cord blood and characterized. ECFCs at passages 3-5 were treated for 24 h with either LPS or vehicle control. Their viability, migration and in vitro vasculogenesis activity were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, modified Boyden chamber and in vitro angiogenesis assays, respectively. ECFC adhesion was assessed by replating cells on fibronectin-coated dishes and subsequent counting of adherent cells.
Results:
Incubation with LPS dose-dependently inhibited the viable, migratory, adhesive and in vitro vasculogenesis capacity of ECFCs.
Conclusion:
LPS impaired the functional activity of ECFCs.
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