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Updated: May 3, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Nitrite reduction in leaves; Studies on isolated chloroplasts
1Department of Plant Science, Faculty of Agriculture, The University, NE1 7RU, Neweastle upon Tyne, UK.
Abstract:
Chloroplast preparations from spinach leaves containing a high percentage of intact chloroplasts were capable of light dependent nitrite reduction at rates around 9 μmol/mg chlorophyll/h for at least 50 min. This reduction was inhibited by DCMU but unaffected by uncouplers of photosynthetic phosphorylation. Nitrite reduction was not accompanied by a stoichiometric evolution of oxygen evolution. The disappearance of nitrite was accompanied by an approximately stoichiometric formation of reduced nitrogen.
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