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Updated: May 3, 2026
In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
Non-coordinate synthesis and methylation of tRNA following excision of plant tissue
1Biology Department, Queen Elizabeth College, W8 7AH, London, UK.
Abstract:
The relationship between the synthesis and methylation of nucleic acids in tissue slices from higher plant storage organs has been investigated. Although the observed nucleic acid synthesis is mainly an expression of rRNA synthesis the highest level of methylation occurs in tRNA. Unlike the synthesis and methylation of rRNA which appears completely coupled, the methylation of tRNA is not tightly coupled to its synthesis. It is suggested that a pool of undermethylated tRNA exists in the tissue prior to incubation and that methylation of this tRNA initially controls the rate of protein synthesis in the tissue slices.
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