Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Roles of Electrolytes: Calcium and Phosphate01:27

Roles of Electrolytes: Calcium and Phosphate

4.6K
Calcium and phosphate are essential electrolytes in the human body, with calcium being the most abundant mineral. Around 99% of the body's calcium is stored in the skeleton and teeth, forming a crystal lattice of mineral salts in combination with phosphates. Calcium plays crucial roles in various bodily functions such as blood clotting, neurotransmitter release, muscle tone maintenance, and nervous and muscle tissue excitability.
The calcium concentration in blood plasma is primarily...
4.6K
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

679
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
679
Pharmacokinetics in Pediatric Patients: Drug Metabolism01:24

Pharmacokinetics in Pediatric Patients: Drug Metabolism

410
In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses...
410
Introduction to Electrolytes01:33

Introduction to Electrolytes

12.5K
In humans, electrolytes play a vital role in various physiological processes. Balancing electrolyte levels is essential for normal body functions; their imbalance can be life-threatening. The major electrolytes include sodium, potassium, chloride, calcium, phosphate, and bicarbonate. They are primarily involved in physiological processes, such as nerve signal transmission, membrane trafficking, muscle contraction, buffering body fluids, and balancing water levels in the body.
Role of Sodium
One...
12.5K
Phosphate Buffer01:22

Phosphate Buffer

5.7K
The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
5.7K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

12.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pulmonary dysfunction is possibly a marker of malnutrition and inflammation but not mortality in patients with end-stage renal disease.

Nephron. Clinical practice·2008
Same author

Ricin toxin B subunit enhancement of rotavirus NSP4 immunogenicity in mice.

Viral immunology·2006
Same author

Synthesis of a ricin toxin B subunit-rotavirus VP7 fusion protein in potato.

Molecular biotechnology·2006
Same author

Bacterial and plant enterotoxin B subunit-autoantigen fusion proteins suppress diabetes insulitis.

Molecular biotechnology·2005
Same author

Synthesis and assembly of a cholera toxin B subunit-rotavirus VP7 fusion protein in transgenic potato.

Molecular biotechnology·2005
Same author

Mucosal immunization with a ricin toxin B subunit-rotavirus NSP4 fusion protein stimulates a Th1 lymphocyte response.

Journal of biotechnology·2005

Related Experiment Video

Updated: May 3, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

9.6K

Kidney and phosphate metabolism.

Nak-Won Choi1

  • 1Depatment of internal Medicine, Konyang University College of Medicine, Daejeon, Korea.

Electrolyte & Blood Pressure : E & BP
|January 25, 2014
PubMed
Summary
This summary is machine-generated.

The kidney regulates serum phosphorus levels using Na+/Pi transporters, influenced by diet, vitamin D, FGF23, and PTH. Alterations in these transporters are linked to phosphate wasting disorders.

Keywords:
PHEXfibroblast growth factor 23phosphorussodium-hydrogen exchanger regulatory factor 1sodium-phosphate cotransporter proteins

More Related Videos

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

1.8K
5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats
05:34

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats

Published on: April 4, 2025

1.8K

Related Experiment Videos

Last Updated: May 3, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

9.6K
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

1.8K
5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats
05:34

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats

Published on: April 4, 2025

1.8K

Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Serum phosphorus homeostasis is crucial for health, involving intestinal absorption, bone storage, and renal reabsorption.
  • The kidney, specifically proximal tubular cells, plays a key role in phosphorus regulation via Na+/Pi transporters.
  • Type IIa and IIc Na+/Pi transporters are vital for renal phosphate reabsorption and are regulated by various factors.

Purpose of the Study:

  • To explore the regulation of renal phosphate reabsorption.
  • To understand the role of Na+/Pi transporters in phosphorus homeostasis.
  • To identify novel genes and mechanisms involved in renal phosphate wasting disorders.

Main Methods:

  • Review of existing literature on renal phosphate transport and regulation.
  • Analysis of the roles of Type IIa and IIc Na+/Pi transporters.
  • Investigation of regulatory factors including dietary phosphate, vitamin D, FGF23, and parathyroid hormone.

Main Results:

  • Type IIa and IIc Na+/Pi transporters are key in renal phosphate reabsorption, with Type IIa responding rapidly to hypophosphatemia and Type IIc more slowly.
  • Altered expression/content of these transporters is associated with physiological and pathophysiological changes in renal Pi reabsorption.
  • PHEX, FGF23, and NHERF1 have been identified as novel regulators in renal phosphate regulation and wasting disorders.

Conclusions:

  • Renal tubular Na+/Pi transporters are central to phosphorus homeostasis.
  • Dysregulation of these transporters and identified genes (PHEX, FGF23, NHERF1) contribute to phosphate wasting disorders.
  • Further research is ongoing to elucidate the precise mechanisms of these novel regulators in renal Pi handling.