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Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

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Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
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Evolutionary Relationships through Genome Comparisons02:54

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
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Cardamine hirsuta: a versatile genetic system for comparative studies.

Angela S Hay1, Bjorn Pieper, Elizabeth Cooke

  • 1Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Köln, Germany.

The Plant Journal : for Cell and Molecular Biology
|January 28, 2014
PubMed
Summary

Cardamine hirsuta offers a new model for studying genetic diversity, closely related to Arabidopsis thaliana. Researchers mapped its genome and identified genetic factors influencing stamen number, aiding evolutionary studies.

Keywords:
Arabidopsis thalianaBrassicaceaeCardamine hirsutacomparative developmentemerging model organismgenetic map

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Area of Science:

  • Genomics and evolutionary biology
  • Plant genetics and breeding

Background:

  • Identifying the genetic basis of phenotypic diversity is crucial for biology, plant breeding, and human genetics.
  • Functional genetic tools are needed to link sequence changes to observed phenotypes.

Purpose of the Study:

  • To establish Cardamine hirsuta as a model system for comparative genetic and transgenic studies.
  • To analyze the genetic basis of phenotypic variation, specifically stamen number, in C. hirsuta.

Main Methods:

  • Development of high-resolution genetic and cytogenetic maps for Cardamine hirsuta.
  • Comparative analysis of morphological and physiological traits with Arabidopsis thaliana.
  • Construction of recombinant inbred lines to detect quantitative trait loci (QTLs) for stamen number.

Main Results:

  • Cardamine hirsuta shares genome structure with the ancestral crucifer karyotype, facilitating comparative genomics.
  • Eight QTLs were identified, explaining variation in stamen number within C. hirsuta populations.
  • Genetic variation in C. hirsuta exhibits clear phylogeographic structure.

Conclusions:

  • Cardamine hirsuta is a valuable experimental system for comparative genetic studies within Brassicaceae.
  • The identified QTLs provide insights into the genetic architecture of stamen number variation.
  • The phylogeographic structure supports evolutionary studies linking genotype, phenotype, and environment.