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Published on: November 29, 2016
Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein
M Milivojevic1, I Petrovic, N Kovacevic-Grujicic
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, 11010, Serbia. isidorapetrovic@imgge.bg.ac.rs.
Abstract:
SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
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