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Updated: May 3, 2026
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Development of an improved time-resolved fluoroimmunoassay for simultaneous quantification of C-peptide and insulin
Tian-Cai Liu1, Mei-Jun Chen1, Zhi-Qi Ren1
1Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Objectives:
Diabetes mellitus is a chronic disease affecting millions of people globally and resulting in significant death rates each year. A fast, inexpensive alternative to traditional testing and monitoring techniques is desirable, since secretion of insulin and C-peptide is impaired in diabetes mellitus.
Design And Methods:
A highly sensitive immunoassay was developed for the simultaneous measurement of C-peptide and insulin levels in human serum, utilizing dual-label time-resolved fluoroimmunoassay (TRFIA) and magnetic particle technologies. This assay was characteristic for a single-step sandwich-type immunoassay, wherein antibody-coated magnetic particles were used as the solid phase and Eu(3+) and Sm(3+) chelate labels were used for detection.
Results:
Antibody-coated magnetic particles in a TRFIA format performed well in addressing a number of quantitative needs.
Conclusions:
The results of this assay correlated well with commercial chemiluminescence assays and provided a number a advantages, including reduced sample volume, reduced reagent and personnel costs and reduced assay time, while maintaining the required clinical sensitivity.
