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Updated: May 3, 2026
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Establishment of a rescue system for an autonomous Parvovirus mink enteritis virus
Daoli Yuan1, Jigui Wang1, Zhili Li1
1State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Abstract:
Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus.
Insights
Researchers created a infectious clone of mink enteritis virus (MEV). The rescued virus matched the original, enabling future studies on MEV replication and host range.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Mink enteritis virus (MEV) is a significant pathogen in mustelids.
- Understanding MEV replication and host tropism is crucial for disease control.
- The lack of a reliable infectious clone has hindered detailed molecular studies.
Purpose of the Study:
- To construct and characterize a full-length infectious clone of the mink enteritis virus.
- To establish a tool for investigating MEV replication, pathogenicity, and host range determinants.
Main Methods:
- Construction of a full-length cDNA clone (pMEV) of the MEV genome.
- Introduction of an engineered BamHI restriction site into the clone as a genetic marker.
- Transfection of feline kidney cells (F81) with the pMEV construct.
- Rescue and characterization of the virus from transfected cells.
Main Results:
- A full-length infectious clone (pMEV) of mink enteritis virus was successfully constructed.
- The rescued virus, distinguished by the engineered BamHI site, was biologically indistinguishable from the parental wild-type virus.
- Transfection and virus rescue were efficiently achieved in feline kidney cells.
Conclusions:
- The developed MEV infectious clone provides a valuable tool for molecular virology research.
- This resource will facilitate in-depth studies into MEV replication mechanisms and pathogenicity.
- Elucidating the determinants of MEV host range is now more feasible with this infectious clone.
