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Silver staining of native and denatured eucaryotic DNA in agarose gels
1Institute of Neurobiology of Slovak Academy of Sciences, Kosice, Czechoslovakia.
Analytical Biochemistry
|August 15, 1987
Abstract:
A modified method of silver staining for native and denatured eucaryotic DNA in 1% agarose gel is described. This method is at least fivefold more sensitive than ethidium bromide staining, with a detection limit of 2.5 ng for total DNA. The calibration curve is linear within the range 5-30 ng of single-stranded and double-stranded DNA. This method is especially advantageous for electrophoretic assessment of DNA molecular weights.