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Updated: May 3, 2026

Sampling Soils in a Heterogeneous Research Plot
Published on: January 7, 2019
Spatial variation in soil properties among North American ecosystems and guidelines for sampling designs
Henry Loescher1, Edward Ayres2, Paul Duffy3
1Science Team, National Ecological Observatory Network (NEON), Boulder, Colorado, United States of America ; Institute of Arctic and Alpine Research (INSTAAR), University of Colorado, Boulder, Colorado, United States of America.
Understanding soil spatial variability is key for accurate ecological studies. This research provides quantitative guidelines for soil sampling strategies, improving sample size and spacing for better data collection across diverse US ecosystems.
Area of Science:
- Soil Science
- Ecology
- Geostatistics
Background:
- Soil properties exhibit significant spatial variability across various scales, complicating accurate mean estimation.
- Effective sampling strategies rely on understanding soil spatial correlation structure, yet current guidelines are often overlooked.
- Logistical constraints frequently dictate sampling designs over quantitative considerations.
Purpose of the Study:
- To assess the spatial variability of soil temperature (Ts) and soil water content (SWC) across diverse US ecosystems.
- To develop quantitative guidelines for optimizing sample size and spacing in soil studies.
- To advance continental-scale understanding of soil spatial behavior.
Main Methods:
- Conducted field assessments at 60 sites representing key US ecosystems.
- Measured soil temperature and water content.
- Utilized semivariograms to quantify spatial variability and correlation structure.
Main Results:
- Developed specific sample size recommendations: e.g., 20 samples for Ts accuracy at temperate/sub-tropical sites, but more for high-latitude sites.
- Soil water content (SWC) demonstrated higher variability than soil temperature (Ts), requiring at least 10x more samples for equivalent accuracy.
- Determined optimal sample spacing for statistical independence across 10 dominant US soil orders.
Conclusions:
- Quantitative guidelines derived from this study enhance the robustness of soil sampling designs.
- Accurate characterization of soil spatial variability is crucial for ecological and biogeochemical research.
- This work provides a continental-scale perspective on soil spatial behavior, informing future research and monitoring efforts.
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