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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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A novel 2006 Indian outbreak strain of Chikungunya virus exhibits different pattern of infection as compared to

Abhishek Kumar1, Prabhudutta Mamidi1, Indrani Das1

  • 1Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, Odisha, India.

Plos One
|January 28, 2014
PubMed
Abstract

Insights

A novel Indian Chikungunya virus (CHIKV) strain, DRDE-06, replicates faster and more efficiently than the prototype S-27 strain. Specific mutations in DRDE-06 may explain its altered infection pattern, warranting further research.

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Chikungunya virus (CHIKV) re-emerged in India after 32 years, causing global epidemics.
  • The unprecedented scale of recent CHIKV outbreaks necessitates understanding viral behavior.

Purpose of the Study:

  • To biologically compare a novel 2006 Indian CHIKV strain (DRDE-06) with the prototype strain (S-27).
  • To investigate the differential infection patterns and replication efficiencies of these CHIKV strains.
  • To identify genetic mutations in DRDE-06 that may influence its characteristics.

Main Methods:

  • Biological comparison of CHIKV strains DRDE-06 and S-27 in mammalian cells.
  • Assays for progeny production, cytopathic effect, viral protein detection, and viral proliferation.
  • Whole-genome mutational analysis of DRDE-06 and comparison with global CHIKV isolates.

Main Results:

  • DRDE-06 demonstrated significantly faster replication and higher progeny production compared to S-27.
  • Mutational analysis revealed 19 mutations in DRDE-06, with 15 consistently present in other outbreak strains.
  • Approximately 46% of recent strains, including DRDE-06, lacked the E1-A226V mutation associated with vector adaptation.

Conclusions:

  • The novel Indian CHIKV strain DRDE-06 exhibits distinct infection patterns and higher replication efficiency than the prototype S-27.
  • Specific genomic mutations in DRDE-06 are likely responsible for its altered biological properties.
  • Further experimental investigation is crucial to elucidate the role of these mutations in CHIKV pathogenesis and adaptation.

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