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Chromosomal polymorphism in the Sporothrix schenckii complex

Alexandre A Sasaki1, Geisa F Fernandes1, Anderson M Rodrigues1

  • 1Discipline of Cellular Biology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil.

Plos One
|January 28, 2014
PubMed

Insights

Genetic diversity in Sporothrix fungi, responsible for sporotrichosis, was explored using pulsed field gel electrophoresis. This study reveals significant chromosomal polymorphisms within and between species, impacting genomic organization.

Area of Science:

  • Medical Mycology
  • Genomics
  • Molecular Biology

Background:

  • Sporotrichosis is a polymorphic fungal infection caused by the *Sporothrix schenckii* complex.
  • Pathogenic species like *S. brasiliensis* and *S. schenckii* sensu stricto (s. str.) pose significant public health challenges.
  • Limited research exists on the genetic polymorphisms and genomic architecture of these fungi.

Purpose of the Study:

  • To investigate chromosomal polymorphisms and genomic organization within the *Sporothrix schenckii* complex.
  • To analyze genetic diversity among different isolates of pathogenic *Sporothrix* species.

Main Methods:

  • Pulsed field gel electrophoresis (PFGE) was employed to separate chromosomal DNA fragments.
  • Nine specific gene loci were mapped onto chromosomal bands using probe hybridization.
  • Brazilian isolates of *S. schenckii* s. str. and *S. brasiliensis* were analyzed.

Main Results:

  • Significant intra- and interspecies polymorphisms in chromosome number and size were observed.
  • Gene hybridization revealed similar genetic organizations in closely related species.
  • Identification of synteny groups in similarly sized chromosomal bands provided insights into genomic structure.

Conclusions:

  • This study provides novel insights into the genetic diversity and genome organization of pathogenic *Sporothrix* species.
  • Understanding chromosomal polymorphisms is crucial for characterizing the *Sporothrix schenckii* complex.
  • Further research into genomic architecture can aid in understanding sporotrichosis epidemiology and pathogenesis.

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