Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

6.3K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

4.3K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interleukin-17A Orchestrates Lung Injury and Remodeling Through p53 and uPA System Crosstalk.

International journal of molecular sciences·2026
Same author

Exposure of <i>Mycobacterium tuberculosis</i> to human alveolar lining fluid shows temporal and strain-specific adaptation to the lung environment.

bioRxiv : the preprint server for biology·2023
Same author

A new tractable method for generating human alveolar macrophage-like cells <i>in vitro</i> to study lung inflammatory processes and diseases.

mBio·2023
Same author

A new tractable method for generating Human Alveolar Macrophage Like cells in vitro to study lung inflammatory processes and diseases.

bioRxiv : the preprint server for biology·2023
Same author

A marmoset model for Mycobacterium avium complex pulmonary disease.

PloS one·2023
Same author

Host- and Age-Dependent Transcriptional Changes in <i>Mycobacterium tuberculosis</i> Cell Envelope Biosynthesis Genes after Exposure to Human Alveolar Lining Fluid.

International journal of molecular sciences·2022

Related Experiment Video

Updated: May 3, 2026

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
07:39

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Published on: June 16, 2018

9.1K

Colony-stimulating factor 1 potentiates lung cancer bone metastasis.

Jaclyn Y Hung1, Diane Horn2, Kathleen Woodruff2

  • 1Division of Hematology and Oncology, Department of Pediatrics, Greehey Children's Cancer Research Institute, University of Texas Health Science Center, San Antonio, TX, USA.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|January 29, 2014
PubMed
Summary

Colony-stimulating factor 1 (CSF1) drives lung cancer bone metastasis by promoting cancer stem cells and osteolysis. Inhibiting CSF1 reduced tumor growth and bone lesions in a mouse model, suggesting new therapeutic strategies.

More Related Videos

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies
09:01

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Published on: July 3, 2025

1.1K
Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
11:41

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Published on: March 15, 2015

11.9K

Related Experiment Videos

Last Updated: May 3, 2026

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
07:39

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Published on: June 16, 2018

9.1K
Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies
09:01

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Published on: July 3, 2025

1.1K
Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
11:41

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Published on: March 15, 2015

11.9K

Area of Science:

  • Cancer Biology
  • Oncology
  • Metastasis Research

Background:

  • Colony-stimulating factor 1 (CSF1) is crucial for osteoclastogenesis and osteolysis in metastatic tumors.
  • Elevated CSF1 levels in lung cancer patients correlate with poor survival and bone metastasis.
  • Lung cancer stem-like cells contribute to tumor growth and metastasis.

Purpose of the Study:

  • To investigate the role of CSF1 in lung cancer bone metastasis.
  • To determine if CSF1 inhibition can ameliorate lung cancer bone metastasis.

Main Methods:

  • Human lung adenocarcinoma A549 cells were analyzed for CSF1 and CSF1 receptor (CSF1R) expression in vitro.
  • A549-luc cells were injected intracardiac into NOD/SCID mice to model bone metastasis.
  • CSF1 knockdown (KD) in A549 cells was achieved using short-hairpin RNA (shRNA) to assess its effect on metastasis.

Main Results:

  • A549 cells express CSF1/CSF1R; CSF1 enhanced proliferation and invasion, while CSF1R inhibition reduced invasion.
  • Intracardiac injection of A549-luc cells led to osteolytic bone lesions and osteoclast activity in mice.
  • CSF1 knockdown reduced CSF1 levels, colony formation, stem-like cell fraction, tumor growth, and osteoclast numbers in vivo.

Conclusions:

  • CSF1 plays a key role in lung cancer stem cell survival and tumor growth, contributing to osteolytic bone metastasis.
  • Inhibition of CSF1 shows potential in reducing lung cancer bone metastasis.
  • This study establishes a relevant preclinical model for lung osteolytic bone metastasis, paving the way for novel therapeutic strategies targeting CSF1.