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Updated: May 3, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Contribution to the structural characterization of eucaryotic PSI reaction centre - II. Characterization of a highly
1Service de Biochimie, Department de Biologie, C.E.N. de SACLAY, BP No2, Gif-sur-Yvette, 91 190, France.
Abstract:
Under precise conditions, SDS PAGE† allows purification of a photoactive P700-chla-protein complex from eucaryotic cells. The yield of P700 recovery is close to 100%. A total protein content equivalent to about 140 kD for one mole of P700 has been estimated by chemical analysis, and electrophoresis revealed the presence of two peptidic chains with MWs close to 65 kD. Photochemical and structural properties of this complex are given and compared with those of other complexes previously isolated.
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