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Rapid lysis technique for mycobacterial species
S S Hurley1, G A Splitter, R A Welch
1Department of Veterinary Science, University of Wisconsin-Madison 53706.
Journal of Clinical Microbiology
|November 1, 1987
Summary
This study introduces an efficient method for lysing mycobacterial cells using a Mini-Beadbeater and zirconium beads. This physical disruption technique yields high-quality DNA and RNA from Mycobacterium paratuberculosis and related species.
Area of Science:
- Microbiology
- Molecular Biology
Background:
- Mycobacterial cell lysis is a challenging and slow process.
- Existing methods like Ribi pressure cell rupture have limitations.
Purpose of the Study:
- To develop a more efficient method for lysing mycobacterial cells.
- To obtain high-quality DNA and RNA from Mycobacteriaceae.
Main Methods:
- Utilized a Mini-Beadbeater cell disrupter.
- Employed zirconium beads for physical disruption.
- Tested on Mycobacterium paratuberculosis and other Mycobacteriaceae.
Main Results:
- Achieved efficient physical rupture of mycobacterial cells.
- Yielded DNA and RNA of high molecular weight.
- Obtained greater quantities of DNA and RNA compared to Ribi pressure cell.
Conclusions:
- The Mini-Beadbeater method offers a superior alternative for mycobacterial cell lysis.
- This technique facilitates higher yields of intact nucleic acids.
- Provides a faster and more effective approach for molecular analysis of mycobacteria.