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Ultrastructural localization of human placental lactogen in distinctive granules in human term placenta: comparison

D W Morrish1, H Marusyk, D Bhardwaj

  • 1Department of Medicine, University of Alberta, Edmonton, Canada.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|February 1, 1988
PubMed

Human placental lactogen (hPL) is known to originate in the syncytiotrophoblast, as demonstrated by light microscopic peroxidase and immunofluorescent staining. However, ultrastructural localization of hPL has not previously been performed. In these experiments, immunostaining of electron microscopic sections using protein A-gold and avidin-biotin complex techniques was used to study hPL and human chorionic gonadotropin (beta hCG) localization in first trimester and term placentae. HPL was localized in many small (0.12-0.25 micron) granules. In contrast, beta hCG was found in large (0.40-1.2 micron) granule complexes. The results therefore demonstrate that these two hormones are stored in two morphologically distinct types of cytoplasmic granules. Since hPL and hCG have different secretory mechanisms, this methodology will be useful in studying these differing mechanisms in human placenta.

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