Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

1.0K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
1.0K
Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

3.8K
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
3.8K
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

1.4K
Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the...
1.4K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.3K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.3K
NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

10.0K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
10.0K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

870
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
870

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell-Free Aragonite-Based Scaffold With Bone Marrow Aspirate Concentrate Augmentation for Osteochondral Defects of the Knee.

Video journal of sports medicine·2025
Same author

Is Glycemic Control and Surgical Approach Related to Surgical Site Infection Following Carpal Tunnel Release?

Journal of hand surgery global online·2025
Same author

Osteochondral Allograft Chondrocyte Viability Is Affected During Harvest by Irrigation Temperature and Reamer Speed.

The American journal of sports medicine·2025
Same author

Saline Irrigation Mitigates Chondrocyte Viability Changes During Trochleoplasty.

The American journal of sports medicine·2025
Same author

Anatomic Drivers of J-Sign Presence and Severity: If There Is a Jump, Look for a Bump.

The American journal of sports medicine·2025
Same author

Long-Term Clinical and Radiographic Outcomes of Meniscus Allograft Transplant.

Current reviews in musculoskeletal medicine·2024

Related Experiment Video

Updated: May 3, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.3K

Proton NMR: a new tool for understanding dissolution.

Steven R Coombes1, Leslie P Hughes, Andrew R Phillips

  • 1Pharmaceutical Development, AstraZeneca , Macclesfield SK10 2NA, United Kingdom.

Analytical Chemistry
|January 30, 2014
PubMed
Summary

Proton Nuclear Magnetic Resonance (NMR) offers superior selectivity and sensitivity for pharmaceutical tablet dissolution analysis compared to UV methods. This technique accurately measures drug and lactose release profiles, revealing consistent dissolution rates.

More Related Videos

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.1K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

4.8K

Related Experiment Videos

Last Updated: May 3, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.3K
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.1K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

4.8K

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Sciences
  • Spectroscopy

Background:

  • Conventional UV spectroscopy for pharmaceutical dissolution testing lacks analyte selectivity and cannot detect non-UV-absorbing compounds.
  • Understanding the dissolution of active pharmaceutical ingredients (APIs) and excipients is crucial for drug product performance.

Purpose of the Study:

  • To introduce and validate Proton Nuclear Magnetic Resonance ((1)H NMR) as an advanced analytical method for pharmaceutical tablet dissolution.
  • To compare the capabilities of (1)H NMR with traditional UV-based dissolution measurements.

Main Methods:

  • Utilized an in-line flow cell coupled with (1)H NMR spectroscopy for real-time dissolution monitoring.
  • Employed water suppression techniques to enhance spectral quality and sensitivity.
  • Performed dissolution studies in a standard pharmacopeial dissolution system using a protic buffer.

Main Results:

  • Achieved high spectral selectivity, with well-resolved signals for multiple analytes (three drugs and lactose) from a single tablet.
  • (1)H NMR demonstrated sufficient sensitivity to quantify compounds present at low concentrations (5 mg).
  • The in-line flow cell provided high-quality NMR spectra with minimal impact on peak shape.

Conclusions:

  • Proton NMR is a powerful, selective, and sensitive technique for comprehensive pharmaceutical tablet dissolution analysis.
  • The method successfully determined the release profiles of both UV-absorbing drugs and non-UV-absorbing lactose.
  • All tested drug substances and lactose exhibited similar relative dissolution rates.