Related Experiment Video
Updated: May 3, 2026
09:46
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
Published on: December 27, 2017
19.4K
Marked changes in platelet count and function following pediatric congenital heart surgery
Jo Bønding Andreasen1, Anne-Mette Hvas, Hanne B Ravn
1Department of Anaesthesiology and Intensive Care, Aarhus University Hospital, Aarhus N, Denmark.
Paediatric Anaesthesia
|January 30, 2014
Summary
Congenital heart surgery using cardiopulmonary bypass (CPB) significantly reduces platelet count and function in children. Longer CPB duration and hypothermia are linked to these adverse effects on platelet activity.
Area of Science:
- Cardiovascular Surgery
- Hematology
- Pediatric Critical Care
Background:
- Reduced hemostatic capacity is a known complication after congenital heart surgery with cardiopulmonary bypass (CPB).
- This hemostatic impairment stems from factors including dilutional coagulopathy and platelet activation within the CPB circuit.
- Understanding platelet behavior post-CPB is crucial for managing surgical outcomes in pediatric patients.
Purpose of the Study:
- To investigate alterations in platelet count and function in pediatric patients undergoing CPB.
- To identify potential correlations between CPB parameters and changes in platelet hemostatic capacity.
Main Methods:
- A prospective, observational study involving 40 children undergoing CPB.
- Platelet count and function were assessed using the Multiplate Analyzer(®) before surgery, immediately after bypass, and on postoperative day 1.
- Conventional coagulation tests and rotational thromboelastometry (ROTEM(®)) were also performed.
Main Results:
- A significant decrease in platelet count was observed immediately after CPB, persisting through postoperative day 1 (P < 0.001).
- Platelet function, measured by ADP and TRAP induction, was significantly reduced post-CPB (P < 0.001 and P = 0.03, respectively).
- CPB duration correlated with decreased platelet count (r = -0.62) and function (r = -0.42 to -0.63). Moderate to deep hypothermia during CPB was associated with reduced platelet function (P = 0.01–0.12).
Conclusions:
- Pediatric patients undergoing corrective heart surgery exhibit significantly reduced platelet count and function after CPB.
- The duration of CPB and the degree of hypothermia are key factors associated with impaired platelet function post-surgery.
Related Concept Videos
01:18Structure and Function of Platelets
7.1K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
7.1K
01:22Formation of the Platelet Plug
6.2K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
6.2K